Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.
Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-lab...
Gardado en:
| Publicado en: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1985
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340973/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2582352/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.1.45 |
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