Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and RNA polymerases in vitro. Polynucleotides cont...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1981
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349103/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6273878/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.11.6633 |
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