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Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(d...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Suggs, S V, Wallace, R B, Hirose, T, Kawashima, E H, Itakura, K
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1981
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349099/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6171820/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.11.6613
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