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A Remote Mutation Affects the Hydride Transfer by Disrupting Concerted Protein Motions in Thymidylate Synthase

The role of protein flexibility in enzyme-catalyzed activation of chemical bonds is an evolving perspective in enzymology. Here we examine the role of protein motions in the hydride transfer reaction catalyzed by thymidylate synthase (TSase). Being remote from the chemical reaction site, the Y209W m...

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Detalhes bibliográficos
Main Authors: Wang, Zhen, Abeysinghe, Thelma, Finer-Moore, Janet S., Stroud, Robert M., Kohen, Amnon
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3490427/
https://ncbi.nlm.nih.gov/pubmed/23034004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja307859m
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