Human somatotropin: covalent reconstitution of two polypeptide contiguous fragments with thrombin.

Thrombin has been shown to resynthesize the Arg134-Thr135 peptide bond between the 134- and 57-residue thrombin fragments of reduced and carbamidomethylated human somatotropin at pH 6.0 in 90% (vol/vol) glycerol. The maximal amount of synthesis was about 20% as estimated by sodium dodecyl sulfate ge...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Gráf, L, Li, C H
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1981
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348992/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6273851/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.10.6135
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