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Human somatotropin: covalent reconstitution of two polypeptide contiguous fragments with thrombin.

Thrombin has been shown to resynthesize the Arg134-Thr135 peptide bond between the 134- and 57-residue thrombin fragments of reduced and carbamidomethylated human somatotropin at pH 6.0 in 90% (vol/vol) glycerol. The maximal amount of synthesis was about 20% as estimated by sodium dodecyl sulfate ge...

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Autors principals: Gráf, L, Li, C H
Format: Artigo
Idioma:Inglês
Publicat: 1981
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC348992/
https://ncbi.nlm.nih.gov/pubmed/6273851
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