Human somatotropin: covalent reconstitution of two polypeptide contiguous fragments with thrombin.
Thrombin has been shown to resynthesize the Arg134-Thr135 peptide bond between the 134- and 57-residue thrombin fragments of reduced and carbamidomethylated human somatotropin at pH 6.0 in 90% (vol/vol) glycerol. The maximal amount of synthesis was about 20% as estimated by sodium dodecyl sulfate ge...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1981
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC348992/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6273851/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.10.6135 |
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