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Synthesis of radiolabeled nicotinamide cofactors from labeled pyridines: versatile probes for enzyme kinetics

(14)C-labeled nicotinamide cofactors are widely employed in biomedical investigations, e.g. to delineate metabolic pathways, to elucidate enzymatic mechanisms, or as substrates in kinetic isotope effect (KIE) experiments. The (14)C-label has generally been located remote from the reactive position,...

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Main Authors: Sen, Arundhuti, Stojković, Vanja, Kohen, Amnon
格式: Artigo
語言:Inglês
出版: 2012
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3472106/
https://ncbi.nlm.nih.gov/pubmed/22922383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.08.012
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