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Construction of a Stability Landscape of the CH3 Domain of Human IgG1 by Combining Directed Evolution with High Throughput Sequencing

One of the most important but still poorly understood issues in protein chemistry is the relationship between sequence and stability of proteins. Here, we present a method for analyzing the influence of each individual residue on the foldability and stability of an entire protein. A randomly mutated...

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Autors principals: Traxlmayr, Michael W., Hasenhindl, Christoph, Hackl, Matthias, Stadlmayr, Gerhard, Rybka, Jakub D., Borth, Nicole, Grillari, Johannes, Rüker, Florian, Obinger, Christian
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3469823/
https://ncbi.nlm.nih.gov/pubmed/22846908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2012.07.017
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