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Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: Application for cell surface antigen detection

The Fc N-glycan chains of four therapeutic monoclonal antibodies (mAbs), namely, Avastin, Rituxan, Remicade, and Herceptin, released by PNGase F, show by MALDI analysis that these biantennary N-glycans are a mixture of G0, G1, and G2 glycoforms. The G0 glycoform has no galactose on the terminal GlcN...

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Bibliografiske detaljer
Main Authors: Boeggeman, Elizabeth, Ramakrishnan, Boopathy, Pasek, Marta, Manzoni, Maria, Puri, Anu, Loomis, Kristin H., Waybright, Timothy J., Qasba, Pradman K.
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3464487/
https://ncbi.nlm.nih.gov/pubmed/19425533
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc900103p
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