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In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins

BACKGROUND: Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways. Glutathione affinity purification is commonly used to isolate and purify glutathione S-transferase (GST)-tagged fusion proteins from total cellular...

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Detalles Bibliográficos
Main Authors: Buhrman, Jason S, Rayahin, Jamie E, Köllmer, Melanie, Gemeinhart, Richard A
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3463477/
https://ncbi.nlm.nih.gov/pubmed/22989306
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-12-63
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