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In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins

BACKGROUND: Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways. Glutathione affinity purification is commonly used to isolate and purify glutathione S-transferase (GST)-tagged fusion proteins from total cellular...

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Main Authors: Buhrman, Jason S, Rayahin, Jamie E, Köllmer, Melanie, Gemeinhart, Richard A
格式: Artigo
語言:Inglês
出版: BioMed Central 2012
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3463477/
https://ncbi.nlm.nih.gov/pubmed/22989306
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-12-63
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