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Purification of Affinity Tag-free Recombinant Tubulin from Insect Cells
α/β-tubulin heterodimers, which can harbor diverse isotypes and post-translational modifications, polymerize into microtubules that are fundamental to many cellular processes. Due to long-standing challenges in generating recombinant tubulin, however, it has been difficult to examine the properties...
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| Published in: | STAR Protoc |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Elsevier
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7416841/ https://ncbi.nlm.nih.gov/pubmed/32783031 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2019.100011 |
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