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In-house preparation of hydrogels for batch affinity purification of glutathione S-transferase tagged recombinant proteins

BACKGROUND: Many branches of biomedical research find use for pure recombinant proteins for direct application or to study other molecules and pathways. Glutathione affinity purification is commonly used to isolate and purify glutathione S-transferase (GST)-tagged fusion proteins from total cellular...

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Detalhes bibliográficos
Main Authors: Buhrman, Jason S, Rayahin, Jamie E, Köllmer, Melanie, Gemeinhart, Richard A
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3463477/
https://ncbi.nlm.nih.gov/pubmed/22989306
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-12-63
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