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Quantitative Measurement of Solvent Accessibility of Histidine Imidazole Groups in Proteins
We report a method to express the solvent accessibility of histidine imidazole groups in proteins. The method is based on measuring the rate of hydrogen exchange (HX) reaction of the imidazole C(ε1)-hydrogen. The rate profile of the HX reaction as a function of pH gives a sigmoidal curve, which reac...
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| Asıl Yazarlar: | , , , , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
2012
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3462353/ https://ncbi.nlm.nih.gov/pubmed/22901083 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi300911d |
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