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Quantitative Measurement of Solvent Accessibility of Histidine Imidazole Groups in Proteins

We report a method to express the solvent accessibility of histidine imidazole groups in proteins. The method is based on measuring the rate of hydrogen exchange (HX) reaction of the imidazole C(ε1)-hydrogen. The rate profile of the HX reaction as a function of pH gives a sigmoidal curve, which reac...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Mullangi, Vennela, Zhou, Xiang, Ball, David W., Anderson, David J., Miyagi, Masaru
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3462353/
https://ncbi.nlm.nih.gov/pubmed/22901083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi300911d
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