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Development and improvement of a serum-free suspension process for the production of recombinant adenoviral vectors using HEK293 cells
Human Embryonic Kidney 293 (HEK293) cells were adapted into a serum-free suspension medium through steps of gradual serum weaning for the production of adenoviral (AdV) gene therapy vectors. The presence of sodium heparin in the medium formulation reduced cell clumping dramatically in suspension cul...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Kluwer Academic Publishers
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3449791/ https://ncbi.nlm.nih.gov/pubmed/19002922 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1020555310558 |
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