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Development and improvement of a serum-free suspension process for the production of recombinant adenoviral vectors using HEK293 cells

Human Embryonic Kidney 293 (HEK293) cells were adapted into a serum-free suspension medium through steps of gradual serum weaning for the production of adenoviral (AdV) gene therapy vectors. The presence of sodium heparin in the medium formulation reduced cell clumping dramatically in suspension cul...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Tsao, Yung-Shyeng, Condon, Russell, Schaefer, Eugene, Lio, Peggy, Liu, Zhong
Format: Artigo
Sprache:Inglês
Veröffentlicht: Kluwer Academic Publishers 2001
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449791/
https://ncbi.nlm.nih.gov/pubmed/19002922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1020555310558
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