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Proteolytic cleavage of recombinant two-chain factor VIII during cell culture production is mediated by protease(s) from lysed cells. The use of pulse labelling directly in production medium

During the production by mammalian cells of recombinant factor VIII from which the B domain was deleted (rFVIII), proteolytic cleavages in the C-terminal part of the heavy chain were observed (Kjalke et al., 1995). By radioactive pulse labelling it was investigated whether the cleavages took place i...

詳細記述

保存先:
書誌詳細
主要な著者: Hansen, Karen, Kjalke, Marianne, Rasmussen, Poul Baad, Kongerslev, Leif, Ezban, Mirella
フォーマット: Artigo
言語:Inglês
出版事項: Kluwer Academic Publishers 1997
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3449617/
https://ncbi.nlm.nih.gov/pubmed/22358766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1023/A:1007988713571
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