Use of an Internal Positive Control in a Multiplex Reverse Transcription-PCR To Detect West Nile Virus RNA in Mosquito Pools

We report on the use of West Nile virus Armored RNA as an internal positive control (IPC) for the extraction and reverse transcription-PCR (RT-PCR) of RNA extracted from field-collected mosquitoes and on a multiplex real-time Taqman RT-PCR to simultaneously detect the 3′ noncoding region of West Nil...

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Bibliografiset tiedot
Päätekijät: Eisler, Diane L., McNabb, Alan, Jorgensen, Danielle R., Isaac-Renton, Judith L.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology 2004
Aiheet:
Virology
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC344474/
https://ncbi.nlm.nih.gov/pubmed/14766868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.2.841-843.2004
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