Use of an Internal Positive Control in a Multiplex Reverse Transcription-PCR To Detect West Nile Virus RNA in Mosquito Pools

We report on the use of West Nile virus Armored RNA as an internal positive control (IPC) for the extraction and reverse transcription-PCR (RT-PCR) of RNA extracted from field-collected mosquitoes and on a multiplex real-time Taqman RT-PCR to simultaneously detect the 3′ noncoding region of West Nil...

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Hlavní autoři: Eisler, Diane L., McNabb, Alan, Jorgensen, Danielle R., Isaac-Renton, Judith L.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2004
Témata:
Virology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC344474/
https://ncbi.nlm.nih.gov/pubmed/14766868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.2.841-843.2004
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