Use of an Internal Positive Control in a Multiplex Reverse Transcription-PCR To Detect West Nile Virus RNA in Mosquito Pools

We report on the use of West Nile virus Armored RNA as an internal positive control (IPC) for the extraction and reverse transcription-PCR (RT-PCR) of RNA extracted from field-collected mosquitoes and on a multiplex real-time Taqman RT-PCR to simultaneously detect the 3′ noncoding region of West Nil...

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Detalhes bibliográficos
Main Authors: Eisler, Diane L., McNabb, Alan, Jorgensen, Danielle R., Isaac-Renton, Judith L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC344474/
https://ncbi.nlm.nih.gov/pubmed/14766868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.2.841-843.2004
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