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Typing and Subtyping Influenza Virus Using DNA Microarrays and Multiplex Reverse Transcriptase PCR

A model DNA microarray has been prepared and shown to facilitate typing and subtyping of human influenza A and B viruses. Reverse transcriptase PCR was used to prepare cDNAs encoding ∼500-bp influenza virus gene fragments, which were then cloned, sequenced, reamplified, and spotted to form a glass-b...

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Autors principals: Li, Jiping, Chen, Shu, Evans, David H.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC87799/
https://ncbi.nlm.nih.gov/pubmed/11158130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.39.2.696-704.2001
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