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Typing and Subtyping Influenza Virus Using DNA Microarrays and Multiplex Reverse Transcriptase PCR

A model DNA microarray has been prepared and shown to facilitate typing and subtyping of human influenza A and B viruses. Reverse transcriptase PCR was used to prepare cDNAs encoding ∼500-bp influenza virus gene fragments, which were then cloned, sequenced, reamplified, and spotted to form a glass-b...

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Vydáno v:J Clin Microbiol
Hlavní autoři: Li, Jiping, Chen, Shu, Evans, David H.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2001
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC87799/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11158130/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.39.2.696-704.2001
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