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Using a Fluorescent Cytosine Analogue tC° to Probe the Effect of Y567 to Ala Substitution on the Pre-insertion Steps of dNMP Incorporation by RB69 DNA Polymerase()

Residues in the Nascent Base-pair binding Pocket (NBP) of bacteriophage RB69 DNA polymerase (RB69pol) are responsible for base discrimination. Replacing Tyr567 with Ala leads to greater flexibility in the NBP, increasing the probability of misincorporation. We used the fluorescent cytosine analogue,...

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Detaylı Bibliyografya
Asıl Yazarlar: Xia, Shuangluo, Beckman, Jeff, Wang, Jimin, Konigsberg, William H.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2012
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3437246/
https://ncbi.nlm.nih.gov/pubmed/22616982
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi300241m
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