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Using a Fluorescent Cytosine Analogue tC° to Probe the Effect of Y567 to Ala Substitution on the Pre-insertion Steps of dNMP Incorporation by RB69 DNA Polymerase()
Residues in the Nascent Base-pair binding Pocket (NBP) of bacteriophage RB69 DNA polymerase (RB69pol) are responsible for base discrimination. Replacing Tyr567 with Ala leads to greater flexibility in the NBP, increasing the probability of misincorporation. We used the fluorescent cytosine analogue,...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3437246/ https://ncbi.nlm.nih.gov/pubmed/22616982 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi300241m |
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