Post-transcriptional Inhibition of Luciferase Reporter Assays by the Nod-like Receptor Proteins NLRX1 and NLRC3

Luciferase reporter assays (LRAs) are widely used to assess the activity of specific signal transduction pathways. Although powerful, rapid and convenient, this technique can also generate artifactual results, as revealed for instance in the case of high throughput screens of inhibitory molecules. H...

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Main Authors: Ling, Arthur, Soares, Fraser, Croitoru, David O., Tattoli, Ivan, Carneiro, Leticia A. M., Boniotto, Michele, Benko, Szilvia, Philpott, Dana J., Girardin, Stephen E.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Biochemistry and Molecular Biology 2012
Fag:
Signal Transduction
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3436573/
https://ncbi.nlm.nih.gov/pubmed/22718770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.333146
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