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Peptide Production and Decay Rates Affect the Quantitative Accuracy of Protein Cleavage Isotope Dilution Mass Spectrometry (PC-IDMS)

No consensus has been reached on the proper time to add stable-isotope labeled (SIL) peptides in protein cleavage isotope dilution mass spectrometry workflows. While quantifying 24 monolignol pathway enzymes in the xylem tissue of Populus trichocarpa, we compared the protein concentrations obtained...

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Bibliografski detalji
Glavni autori: Shuford, Christopher M., Sederoff, Ronald R., Chiang, Vincent L., Muddiman, David C.
Format: Artigo
Jezik:Inglês
Izdano: The American Society for Biochemistry and Molecular Biology 2012
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3434783/
https://ncbi.nlm.nih.gov/pubmed/22595788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O112.017145
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