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Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules
We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequence...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2012
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3433648/ https://ncbi.nlm.nih.gov/pubmed/22886093 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2137 |
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