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Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules

We present dial-out PCR, a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequence...

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Detalhes bibliográficos
Main Authors: Schwartz, Jerrod J, Lee, Choli, Shendure, Jay
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3433648/
https://ncbi.nlm.nih.gov/pubmed/22886093
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2137
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