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Direct observation of mammalian cell growth and size regulation

We introduce a microfluidic system for simultaneously measuring single cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 hours of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in...

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Main Authors: Son, Sungmin, Tzur, Amit, Weng, Yaochung, Jorgensen, Paul, Kim, Jisoo, Kirschner, Marc W., Manalis, Scott R.
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3433595/
https://ncbi.nlm.nih.gov/pubmed/22863882
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2133
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