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Crystallization and preliminary X-ray crystallographic analysis of Aquifex aeolicus SelA, a bacterial selenocysteine synthase

Selenocysteine (Sec), the 21st amino acid, is synthesized on its specific tRNA (tRNA(Sec)) via a multi-step process. In bacteria, tRNA(Sec) is ligated first with serine by seryl-tRNA synthetase, which is followed by Ser-to-Sec conversion by Sec synthase (SelA). To elucidate its structure and catalyt...

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Bibliographic Details
Main Authors: Itoh, Yuzuru, Sekine, Shun-ichi, Yokoyama, Shigeyuki
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3433215/
https://ncbi.nlm.nih.gov/pubmed/22949212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112033519
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