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Efficient reverse transcription of cowpea mosaic virus RNAs.
Conditions are described which give an efficient synthesis of DNA copies of cowpea mosaic virus (CPMV) RNAs, using avian myeloblastosis reverse transcriptase and oligo (dT) primers. Maximum incorporation of dAMP into cDNA is attained with 0.4 to 0.8 mM of each deoxynucleoside triphosphate, 12 mM Mg+...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1978
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342779/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/85293/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.12.4643 |
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