Efficient reverse transcription of cowpea mosaic virus RNAs.

Conditions are described which give an efficient synthesis of DNA copies of cowpea mosaic virus (CPMV) RNAs, using avian myeloblastosis reverse transcriptase and oligo (dT) primers. Maximum incorporation of dAMP into cDNA is attained with 0.4 to 0.8 mM of each deoxynucleoside triphosphate, 12 mM Mg+...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Davies, J W, Verver, J W, Goldbach, R W, Van Kammen, A
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 1978
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342779/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/85293/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.12.4643
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