Efficient reverse transcription of cowpea mosaic virus RNAs.

Conditions are described which give an efficient synthesis of DNA copies of cowpea mosaic virus (CPMV) RNAs, using avian myeloblastosis reverse transcriptase and oligo (dT) primers. Maximum incorporation of dAMP into cDNA is attained with 0.4 to 0.8 mM of each deoxynucleoside triphosphate, 12 mM Mg+...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Davies, J W, Verver, J W, Goldbach, R W, Van Kammen, A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1978
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342779/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/85293/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.12.4643
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