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Efficient reverse transcription of cowpea mosaic virus RNAs.

Conditions are described which give an efficient synthesis of DNA copies of cowpea mosaic virus (CPMV) RNAs, using avian myeloblastosis reverse transcriptase and oligo (dT) primers. Maximum incorporation of dAMP into cDNA is attained with 0.4 to 0.8 mM of each deoxynucleoside triphosphate, 12 mM Mg+...

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Bibliografiska uppgifter
I publikationen:Nucleic Acids Res
Huvudupphov: Davies, J W, Verver, J W, Goldbach, R W, Van Kammen, A
Materialtyp: Artigo
Språk:Inglês
Utgiven: Oxford University Press 1978
Länkar:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342779/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/85293/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.12.4643
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