Separation of ribosomal subunits of Escherichia coli by Sepharose chromatography using reverse salt gradient.

A mixture of 30 S and 50 S subunits quantitatively absorbs on a column of Sepharose--4B from the buffer: 0.02 M Tris--HCl, pH 7.5, containing 1.5 M (NH4)2SO4. During elution by reverse gradient of ammonium sulphate (1.5--0.05 M) the subunits are eluted at different salt concentrations. Complete sepa...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Kirillov, S V, Makhno, V I, Peshin, N N, Semenkov, Y P
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1978
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342750/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/364425/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.11.4305
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