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Mechanism of codon-anticodon interaction in ribosomes. Direct functional evidence that isolated 30S subunits contain two codon-specific binding sites for transfer RNA.

30S subunits were isolated capable to bind simultaneously two molecules of Phe-tRNAPhe (or N-Acetyl-Phe-tRNAPhe), both poly(U) dependent. The site with higher affinity to tRNA was identified as P site. tRNA binding to this site was not inhibited by low concentrations of tetracycline (2 x 10(-5)M) an...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Kirillov, S V, Makhno, V I, Semenkov, Y P
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1980
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC327251/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6986612/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/8.1.183
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