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Mechanism of codon-anticodon interaction in ribosomes. Direct functional evidence that isolated 30S subunits contain two codon-specific binding sites for transfer RNA.

30S subunits were isolated capable to bind simultaneously two molecules of Phe-tRNAPhe (or N-Acetyl-Phe-tRNAPhe), both poly(U) dependent. The site with higher affinity to tRNA was identified as P site. tRNA binding to this site was not inhibited by low concentrations of tetracycline (2 x 10(-5)M) an...

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Detalles Bibliográficos
Main Authors: Kirillov, S V, Makhno, V I, Semenkov, Y P
Formato: Artigo
Idioma:Inglês
Publicado: 1980
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC327251/
https://ncbi.nlm.nih.gov/pubmed/6986612
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