Separation of ribosomal subunits of Escherichia coli by Sepharose chromatography using reverse salt gradient.
A mixture of 30 S and 50 S subunits quantitatively absorbs on a column of Sepharose--4B from the buffer: 0.02 M Tris--HCl, pH 7.5, containing 1.5 M (NH4)2SO4. During elution by reverse gradient of ammonium sulphate (1.5--0.05 M) the subunits are eluted at different salt concentrations. Complete sepa...
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | Nucleic Acids Res |
|---|---|
| Prif Awduron: | , , , |
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
Oxford University Press
1978
|
| Mynediad Ar-lein: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342750/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/364425/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.11.4305 |
| Tagiau: |
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!
|