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Substrate dependence of the mechanism of EcoRI endonuclease.

The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double st...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Principais autores: Rubin, R A, Modrich, P
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1978
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342221/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/211492/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.8.2991
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