Substrate dependence of the mechanism of EcoRI endonuclease.
The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double st...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1978
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342221/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/211492/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.8.2991 |
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