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Substrate dependence of the mechanism of EcoRI endonuclease.
The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double st...
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| 主要な著者: | , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
1978
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342221/ https://ncbi.nlm.nih.gov/pubmed/211492 |
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