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Preparation and isolation of covalently closed circular rDNA molecules from DNA of Xenopus laevis.
We describe a method leading to the formation of closed circles of rDNA starting from total DNA of Xenopus laevis. Linear DNA molecules were digested with exonuclease 3 and self-annealed. Open circles were enriched and covalently closed by the simultaneous use of polynucleotide kinase, DNA polymeras...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1978
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342154/ https://ncbi.nlm.nih.gov/pubmed/673851 |
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