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Preparation and isolation of covalently closed circular rDNA molecules from DNA of Xenopus laevis.

We describe a method leading to the formation of closed circles of rDNA starting from total DNA of Xenopus laevis. Linear DNA molecules were digested with exonuclease 3 and self-annealed. Open circles were enriched and covalently closed by the simultaneous use of polynucleotide kinase, DNA polymeras...

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書目詳細資料
Main Authors: Polito, L C, Spadari, S
格式: Artigo
語言:Inglês
出版: 1978
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC342154/
https://ncbi.nlm.nih.gov/pubmed/673851
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