Preparation and isolation of covalently closed circular rDNA molecules from DNA of Xenopus laevis.
We describe a method leading to the formation of closed circles of rDNA starting from total DNA of Xenopus laevis. Linear DNA molecules were digested with exonuclease 3 and self-annealed. Open circles were enriched and covalently closed by the simultaneous use of polynucleotide kinase, DNA polymeras...
Gespeichert in:
| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1978
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342154/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/673851/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.6.2211 |
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