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Preparation and isolation of covalently closed circular rDNA molecules from DNA of Xenopus laevis.

We describe a method leading to the formation of closed circles of rDNA starting from total DNA of Xenopus laevis. Linear DNA molecules were digested with exonuclease 3 and self-annealed. Open circles were enriched and covalently closed by the simultaneous use of polynucleotide kinase, DNA polymeras...

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Detalhes bibliográficos
Main Authors: Polito, L C, Spadari, S
Formato: Artigo
Idioma:Inglês
Publicado em: 1978
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC342154/
https://ncbi.nlm.nih.gov/pubmed/673851
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