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In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state

EmrE is a small multidrug transporter from Escherichia coli that provides a unique model for the study of polytopic membrane proteins. Here, we show its synthesis in a cell-free system in a fully functional form. The detergent-solubilized protein binds substrates with high affinity and, when reconst...

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Autors principals: Elbaz, Yael, Steiner-Mordoch, Sonia, Danieli, Tsafi, Schuldiner, Shimon
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC341767/
https://ncbi.nlm.nih.gov/pubmed/14755055
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0306533101
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