Nalaganje...
In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state
EmrE is a small multidrug transporter from Escherichia coli that provides a unique model for the study of polytopic membrane proteins. Here, we show its synthesis in a cell-free system in a fully functional form. The detergent-solubilized protein binds substrates with high affinity and, when reconst...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
2004
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC341767/ https://ncbi.nlm.nih.gov/pubmed/14755055 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0306533101 |
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