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Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

BACKGROUND: Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and within certain distances that allo...

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Bibliografiset tiedot
Päätekijät: Ye, Jian, Coulouris, George, Zaretskaya, Irena, Cutcutache, Ioana, Rozen, Steve, Madden, Thomas L
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: BioMed Central 2012
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3412702/
https://ncbi.nlm.nih.gov/pubmed/22708584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-13-134
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