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Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction
BACKGROUND: Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and within certain distances that allo...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3412702/ https://ncbi.nlm.nih.gov/pubmed/22708584 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-13-134 |
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