Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

BACKGROUND: Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and within certain distances that allo...

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Bibliographic Details
Main Authors: Ye, Jian, Coulouris, George, Zaretskaya, Irena, Cutcutache, Ioana, Rozen, Steve, Madden, Thomas L
Format: Artigo
Language:Inglês
Published: BioMed Central 2012
Subjects:
Software
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3412702/
https://ncbi.nlm.nih.gov/pubmed/22708584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-13-134
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