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Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

BACKGROUND: Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and within certain distances that allo...

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Autors principals: Ye, Jian, Coulouris, George, Zaretskaya, Irena, Cutcutache, Ioana, Rozen, Steve, Madden, Thomas L
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3412702/
https://ncbi.nlm.nih.gov/pubmed/22708584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-13-134
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